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WebA method for expressing a recombinant protein containing unnatural amino acids in animal cells. On the basis of four groups of aminoacyl-tRNA synthetases and corresponding tRNAs, aminoacyl-tRNAs that recognize stop codons are constructed, and three gene codon expansion systems are obtained by screening. The three gene codon expansion systems … Webthermo-shaker and incubated at 95 °C for 10 mins with shaking at 1000g. The plate was cooled to room temperature and 1 µg of trypsin was added to each sample. The mixture was incubated for 1 hour at 50 °C. From the mixture 140 µL of the supernatant was transferred to a sample vial. LC Separation: Separation was accomplished using a WebFeb 12, 2013 · Designed to make LC-MS/MS analysis accessible to more clinical researchers. Thermo Fisher Scientific, Inc. has introduced the Thermo Scientific Prelude … ehrm strategic review