WebpAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA (Plasmid #74513) Print Enlarge View all sequences Purpose AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide. Depositing Lab Guoping Feng Publication Web(pSLenti‑SFH‑EGFP‑P2A‑Puro‑CMV‑THBS1‑3xFLAG‑WPRE). The third lentivirus generation system was used and the interim 293T cell line (ATCC) was used. The quantity of lentiviral plasmid used for transfection was 10 µg in a 10 cm cell culture dish, and the ratio of the lentiviral plasmid: pack‑ aging vector: envelope was 0.25:1:0.75.

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WebIn the Pim-1 OE group, recombinant lentivirus vector (pSLenti-EF1-EGFP-P2A-Puro-CMV-Pim-1-3xFLAG-WPRE) and 15μL Polybrene (1mg/mL) were added. The volume of lentivirus required was calculated according to the multiplicity of infection (MOI) value obtained from a pre-experiment. WebMar 29, 2024 · pIRES2-EGFP vector was used to express HNF4α (Tsingke Biotechnology, Beijing). The lentiviral pLVX-puro vector was used to express OATP2B1, and the lentiviral … roc the show cast

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Webthe pSLenti-SFH-EGFR-P2A-Puro-CMV-3xFLAG-WPRE vector. The lentivirus was purchased from OBIO Technology (Shanghai, China). For infection, cells were stably trans-fected with the lentivirus. Afterward, the cells were cul-tured in medium containing puromycin dihydrochloride (MedChemExpress). Finally, monoclonal stable transgenic cell WebSep 5, 2024 · All lentiviral vectors were purchased from OBiO Technology (Shanghai, China). The pSLenti-SFH-EGFP-P2A-Puro-CMV-Nt5e-WPRE lentiviral vectors ware transfected into B16-F10 cells. The transfected cells were screened with puromycin (2 μg/ml) for 1 week to establish stable cell lines. In vivo assays WebpLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus Reliably co-label cells with rFLuc and GFP driven by a moderate EF1α promoter (and with puro selection) - great for tracking cells, HTS assays, and more Proven—leverages SBI’s third generation lentivector technology for optimal virus titers roc the royal